Kaolinite-glucose intercalation compound was synthesized by the intercalation of polar organic big molecule dodecylamine and then displaced by glucose in the layer of kaolinite. XRD, FT-IR, TG and DSC were used to study the reaction process and the characters of the products. The results indicated that the basal spacing of kaolinite increased to 2.280 nm from 0.717 nm, and the hydrogen bonds were formed between dodecylamine NH and kaolinite Si-O after dodecylamine intercalation. Kaolinite-dodecylamine intercalation compound was stable below 295 °C. After displacement reaction by glucose, the basal spacing of kaolinite increased to 3.364 nm, and the intercalation rate reached 87%, the hydrogen bonds were formed between glucose C=O and kaolinite inner surface hydroxyl. Kaolinite-glucose intercalation compound was stable below 302 °C.