A structural steel should satisfy various properties under varied conditions. Fatigue strength is one of them. When structural steels are used in the form of welded joints, fatigue strength is one of the key characteristics that should be considered. In this study, comprehensive fatigue tests of a structural steel with yielding strength of about 350 MPa and tensile strength of about 520 MPa were carried out. First, a lot of specimens: parent material plates, butt welded specimens with reinforcement removed, as-welded plates, plates with weld toe ground, load-carrying cruciform, non load-carrying cruciform, plates with transverse fillet welded rib, etc were prepared and tested. S-N curves for the above specimens were compared and analyzed. Secondly, some life-size rectangular beams were fabricated by welding and tested. It is found that annealing is detrimental to fatigue strength rather than beneficial. The experimental data and results may be used usefully by engineers.