In this study, the influence of electrolytic composition and time deposition parameters on the quality of titanium oxide coating was evaluated. The commercial Ti discs were anodized by micro-arc oxidation (MAO) process using calcium acetate monohydrate 0.5M (CaAc) and a mixture of calcium acetate monohydrate 0.5M and sodium beta-glycerophosphate 0.02M (CaAc + BGF) aqueous solutions. MAO was carried out by applying pulsed DC voltage of 150 V at different processing time (05, 15, 30, 45 and 60 seconds). The films were characterized by SEM, EDS, surface profilometer and XRD. Additionally, osteoblasts culture was carried out in order to verify the cytocompatibility of the anodized surfaces. The results showed the formation a well controlled homogenous microporous structures with irregular shapes for both electrolytes. Presence of Ca and P on the surface was found for films produced with CaAc + BGF electrolyte. Also, the adhesion, spreading, proliferation, and differentiation of osteoblast cells were very similar.