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Surface Analysis and Cell Biology Technique in Understanding Degradation of Natural Temporary Teeth Collected from an Area with High Pollution

Journal Key Engineering Materials (Volumes 396 - 398)
Volume Bioceramics 21
Edited by Marcelo Prado and CecĂ­lia Zavaglia
Pages 35-38
DOI 10.4028/www.scientific.net/KEM.396-398.35
Citation Ioana Demetrescu et al., 2008, Key Engineering Materials, 396-398, 35
Online since October, 2008
Authors Ioana Demetrescu, D. Iordachescu, D. Ionita, Stef Manea
Keywords Cell Culture, Hydrophilic-Hydrophobic Balance, SEM Analysis, Temporary Teeth
Abstract

The aim of this paper is to correlate surface features of degraded temporary teeth from area with high pollution with the cell adhesion and proliferation. Viability of gingival fibroblast (HGF-1, CRL-2014, American Type Culture Collection) was evaluated with a MTT (3-(4,5-dimetiltiazol-2-il)-2,5-difeniltetrazolium bromide ) test and the values are discussed for the most degraded temporary teeth in correlation with the change in surface morphology and hydrophilic/hydrophobic balance, taking into account that cell adhesion is related to a more hydrophilic character.

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