In this study, commercially pure titanium (Ti-cp) sheets grade 2 were used as substrates. To investigate the role of composition and characteristics of titanium surface oxides in cellular behavior of osteoblasts, the surface of titanium were modified by anodic oxidation with sulphuric acid. Subsequently were treated part of anodized samples with FN and cultured with osteoblast cells for 30, 120 and 240 minutes. The chemical composition and topography have influenced the interaction between fibronectin and substrate. Cells were found more associated to the surfaces that have been submitted to anodization and FN coating than to the related controls.