Paper Title:
A Collagen Grafting on Hydroxyapatite to Enhancement of Bone Cell Attachment
  Abstract

A collagen material was chemically grafted on hydroxyapatite (HA) to enhance bone cell attachment because the collagen is a major constituent of connective tissues and has been regarded as one of the most excellent coating materials for bone bonding. First, HA disks were prepared with 12mm diameter and 1mm thickness. And then collagen (type I) was immobilbized on the HA surface using a 3-APTES coupling agent on HA disk surfaces. MC3T3-E1 osteoblasts were seeded on the collagen-grafted and non-grated HA disks and cultured for 4 hrs to evaluate the cell adhesion on the HA discs. The Attached cell morphology on discs was observed with a fluorescent optical microscopy (FOM) and a scanning electron microscopy (SEM). The osteoblasts on the collagen-grafted sample were more spread than those on the non-grafted sample. It is believed that collagen-grafted HA surface provides suitable sites for cell attaching due to the high biocompatibility of collagen.

  Info
Periodical
Key Engineering Materials (Volumes 396-398)
Main Theme
Edited by
Marcelo Prado and Cecília Zavaglia
Pages
41-45
DOI
10.4028/www.scientific.net/KEM.396-398.41
Citation
D.W. Lee, E.J. Lee, S. S. Chun, M. W. Ahn, I.W. Song, I.K. Kang, S. Y. Kim, "A Collagen Grafting on Hydroxyapatite to Enhancement of Bone Cell Attachment", Key Engineering Materials, Vols. 396-398, pp. 41-45, 2009
Online since
October 2008
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Price
$32.00
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