Paper Title:
Synthesis and Biocompatibility of Hydroxyapatite in a Graphite Oxide Matrix
  Abstract

Though hydroxyapatite has the ability to promote bone growing, devices based on OHAp are mechanically weak and need to be reinforced for load bearing applications or in the manufacturing of scaffolds for bone regeneration. Graphite (Gr) could provide appropriate reinforcement properties to OHAp without being deleterious for the biocompatibility of the system. This paper describes an accelerated synthesis of the OHAp with ultrasonic agitation in the presence of functionalized graphite (GO). The toxicity of the Graphite and the GO-OHAp system is evaluated. GO-OHAp was produced by a wet chemical reaction involving CaCl2 and Na2HPO4. The calcium salt solution was added first and the solution sonicated for 1 hour, before repeating the operation with the phosphate solution. Biocompatibility was tested by using a primary cell culture of HOB (ECCAC). The disappearance of the maximum at 2q = 26.32º corresponding to the d002 plane of graphite and the appearance of the maximum at 2q = 13.2º in the XRD patterns is related with an expansion of the grapheme sheets from 0.34 nm to 0.59 nm and it has been used to assess the graphite oxidation. The OHAP on GO growing has been confirmed by the appearance of a broad peak centred at 2q = 31.5º and a sharpened peak at 2q = 26.0º characteristic of low crystalline apatites. Although the employed graphite can be considered biocompatible, cellular viability is significantly improved by the presence of apatite.

  Info
Periodical
Key Engineering Materials (Volumes 396-398)
Main Theme
Edited by
Marcelo Prado and Cecília Zavaglia
Pages
477-480
DOI
10.4028/www.scientific.net/KEM.396-398.477
Citation
L. M. Rodríguez-Lorenzo, L. Benito-Garzón, F. Barroso-Bujans, M. Fernández, "Synthesis and Biocompatibility of Hydroxyapatite in a Graphite Oxide Matrix", Key Engineering Materials, Vols. 396-398, pp. 477-480, 2009
Online since
October 2008
Export
Price
$32.00
Share

In order to see related information, you need to Login.

In order to see related information, you need to Login.

Authors: Miho Tanuma, Yoshikazu Kameshima, Akira Nakajima, Kiyoshi Okada, Shigeo Asai, Masao Sumita
Abstract:We have reported that CaSiO3 ceramics show very fast bone-like apatite formation in simulated body fluid (SBF). However, CaSiO3 ceramics have...
1161
Authors: S. Chun, D.H. Shin, I.K. Kang, M.W. Ahn, Suk Young Kim
Abstract:In order to enhance bone cell adhesion on hydroxyapatite (HA), collagen was used as a surface-grafting material on HA substrates because the...
1143
Authors: Han Guo, Jie Wei, Hang Kong, Chang Sheng Liu, Ke Feng Pan
Abstract:Porous calcium phosphate cement (CPC) scaffolds were successfully fabricated utilizing particle-leaching method. Mesenchymal stem cells...
1383
Authors: Wei Bin Zhang, Wai Ching Liu, Wei Ming Gu, Lei Chen, Yu Hui Shen
Chapter 1: Material Science
Abstract:Calcium silicate (CS), the recent developed biodegradable ceramic, shows potential in stimulating bone regeneration. However, the rapid...
195
Authors: Kun Mediaswanti, Cui'e Wen, Elena P. Ivanova, Christopher C. Berndt, Francois Malherbe, James Wang
Chapter 9: New Functional Materials
Abstract:Titanium and titanium alloys scaffolds have been widely explored in many load-bearing orthopaedic applications due to their excellent...
1634