Paper Title:
Photo-Oxidative Stress on Model Membranes – Studies by Optical Methods
  Abstract

Photophysical studies on oxidative stress induced by illumination with ultraviolet (UV) light from artificial light sources, were performed on small unilamellar liposomes (SUVs) as models of biomembranes. Different liposoluble antioxidants were incorporated in the liposome bilayer during the lipid film preparation. The quercetin containing bilayers exhibited a high tolerance to photooxidative stress. The light-driven reactive oxygen species (ROS) effects on liposomal membranes were monitored by optical methods: UV-VIS absorption, fluorescence and chemiluminescence (CL), exploiting the spectral properties of chlorophyll a (Chla) embedded into some liposome bilayers (Chla/lipid molar ratio = 1/100). The interaction between Chla and the other liposome components resulted in changes in their absorption and emission fluorescence properties.

  Info
Periodical
Edited by
Daniela Ionita
Pages
29-32
DOI
10.4028/www.scientific.net/KEM.415.29
Citation
M. E. Bărbînţă Pătraşcu, N. M. Badea, L. Ţugulea, A. Meghea, "Photo-Oxidative Stress on Model Membranes – Studies by Optical Methods", Key Engineering Materials, Vol. 415, pp. 29-32, 2009
Online since
September 2009
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$32.00
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