Paper Title:
Dynamics of Dental Pellicle Formation - In Vitro Analysis of Time Dependant Binding Behavior by Surface Plasmon Resonance and the Influence of Oral Therapeutics
  Abstract

: The pellicle on oral surfaces represents a central interface for the formation of biofilms. Among other things it causes the first adsorption of bacteria. The dynamics of pellicle formation, on tooth surfaces and the influence of oral therapeutics on the pellicle structure are fairly unknown. With the method of surface plasmon resonance (SPR), the formation of salivary pellicle structures on hydroxylapatite (HAP) surfaces covering a very thin (~50nm) layer gold on a glass prism was recorded in real time without labeling or destruction. As pellicle forming substrates natural pooled human saliva (NS) and artificial saliva (AS) were used. To simulate the influence of therapeutic additives on the dynamic of the pellicle forming process, a chlorhexidine preparate (Chlorhexamed Fluid® CHX) on two different concentrations was selected. The binding behavior of a NS and a preparation in terms of an AS were compared. The layer was largely stable against rinsing with buffer. The application of CHX preparations in two different concentrations as an example of an oral therapeutic additive revealed a complex dynamic of adsorption. CHX did not lead to any visible destruction of the pellicle. The introduced method is an excellent tool to illustrate the dynamic effects of pellicle formation or pellicle reorganization by measuring the increase or decrease of the SPR signal in real time.

  Info
Periodical
Edited by
Daniela Ionita
Pages
77-80
DOI
10.4028/www.scientific.net/KEM.415.77
Citation
D. Vornicescu, K. Solanska, I. Demetrescu, M. Frentzen, M. Keusgen, "Dynamics of Dental Pellicle Formation - In Vitro Analysis of Time Dependant Binding Behavior by Surface Plasmon Resonance and the Influence of Oral Therapeutics", Key Engineering Materials, Vol. 415, pp. 77-80, 2009
Online since
September 2009
Export
Price
$35.00
Share

In order to see related information, you need to Login.

In order to see related information, you need to Login.

Authors: B. Şermet, S. Değer, M.C. Balkaya, D. Kaya, G. Külekçi
2023
Authors: Suck Jin Hong, B.I. Kim, H.K. Kwon, Choong Ho Choi
Abstract:The purpose of this study was to investigate the remineralization effect of Galla Rhois and Psoralea corylifolia extracts with sodium...
937
Authors: Huang Qin Chen, Bin Huang
Chapter 1: Materials Science and Materials Processing Technology
Abstract:The purpose of this study was to investigate the effect of polyanionsodium tripolyphosphate (STP) on color stability of the bleached enamel...
57
Authors: Sroisiri Thaweboon, Yadana Aung, Suwannee Luppanapornlarp, Supatchai Boonpratham, Boonyanit Thaweboon
Chapter 5: Biomaterials
Abstract:Objective: The aim of this study was to evaluate the adhesion of Streptococcus mutans (S. mutans) on different types of orthodontic...
467
Authors: Pavel Topala, Vitalie Besliu, Laurentiu Marin
Chapter 1: Applied Materials and Technologies
Abstract:The paper aims at studying the phenomenon of adhesion between the graphite pellicles deposited through electric discharges in pulse (EDI)...
172