The modern steel orthotropic decks have been used in steel bridges for 60 years all over the world because of its super structural advantages. Recently, more bridge owners, engineers and researchers pay more attention to the fatigue problem of orthotropic steel decks for a large number of fatigue cracks found in steel bridges. For example, bridge engineers have detected hundreds of fatigue cracks in steel orthotropic deck on the 888-meter long box girder of Humen Bridge only ten years after opening to traffic. How to design or repair the fatigue details in orthotropic steel decks is the critical question to be solved at first step. In current paper, the elaborate numerical analysis model of the orthotropic steel bridge decks was developed using ANSYS software with different floor-beam web cutouts shapes, such as conventional ellipse, circular, trapezoid and Haibach web cutouts. The finite element models were calibrated by static test of one full size orthotropic steel bridge deck model. According to the analysis results, it should select the rational cutout shapes based on actual load and structural conditions in steel bridge deck design and strengthening.