This work presents a study of a fatigue test and a finite element analysis on an arch bridge stainless steel suspender with threaded connections. A suspender which had a diameter of 70mm was tested under axial tensile loads range from 430kN to 700kN. The suspender was sudden failure from the thread root of the first loaded tooth in the pin after 1546609 cycles. Then, a two-dimensional axisymmetric modeling ignoring the helix angle of the thread was established with finite element software ANSYS to perform a stress analysis of the threaded connection. The stress concentration factors (SCFs) at the root of the teeth of pin were investigated under the applied external loading. The conclusive results had been drawn from the analysis including the location and the value of maximum SCF in the pin. Finally, the location and the value of the maximum von Mises stress were given. The results showed that the location of the fracture surface was consistent with the location of the maximum von Mises stress.