This work has been undertaken to study the microfluidic chip made by the excimer laser ablation. A Kind of Micro electrophoresis chip, Micro polymer chain reaction (PCR) and combined with electrochemical detection and micro blood separator are made using the commercial Polyimide film (Kapton from Dupont). The surface characterization of the excimer-ablated polymide is studied by using the Scanning Electron Microscopy (SEM), Atomic Force Microscope (AFM) and X-ray photoelectronic spectroscopy (XPS). The surface roughness is also measured by white light interference microscope (Wyco NT8000, Vecco. Com, USA). A kind of heat transfer equation is also given in this paper.