Paper Title:
The Hydrophobicity of Micro Water Pool and the Interfacial Fluidity of PC-Based W/O Microemulsion Droplet Involved with Reactivity of Phospholipase A2
  Abstract

The impact of physicochemical character of the W/O microemulsion droplet on the reactivity of phospholipase A2 (PLA2) was investigated for optimal design of the micro-reactor. Hydrophobicity of micro water pool and fluidity of micro-scaled interface of W/O microemulsion droplet were dominant factors to determine the appearance of maximum reactivity. Phosphatidylcholine (PC) in this system performed not only as a substrate for PLA2 but also as an amphiphilic molecule to form W/O microemulsion droplet. The organic phase was composed by isooctane (C8*) as a main solvent and 1-butanol (C4) as a co-solvent. The molar ratio was fixed as isooctane:1-butanol =11:1. The water content in the W/O microemulsion was indicated by the molar ratio of H2O moles to PC moles presented by Wsoln [-](≡[mol-H2Osoln]/[mol-PC]). By the increasing of the water content from 1 to 5, the reactivity of PLA2 was remarkably increased from 0.01 to 0.03 [mM・s-1・mg-PLA2-1]. Over 5 of Wsoln range, the reactivity was decreased. The optimal water content was indicated as Wsoln=5 [-]. The hydrophilicity of micro water pool and the interfacial fluidity of the water pool were detected by the signal of the fluorescence probes, Coumarin 343 and TMA-DPH, respectively. The hydrophobicity was decayed with the increasing of the water content. More than 10 of Wsoln, the hydrophobicity was fully decayed and achieved to bulk aqueous one. The interfacial fluidity was decreased with the increasing of the water content. In more than Wsoln of 5, the reactivity was decreased by the decline of the collision frequency between PLA2 and PC molecule due to lower fluidity of micro-scaled interface.

  Info
Periodical
Edited by
Osamu Hanaizumi and Masafumi Unno
Pages
100-108
DOI
10.4028/www.scientific.net/KEM.459.100
Citation
K. Yamazaki, M. Imai, "The Hydrophobicity of Micro Water Pool and the Interfacial Fluidity of PC-Based W/O Microemulsion Droplet Involved with Reactivity of Phospholipase A2", Key Engineering Materials, Vol. 459, pp. 100-108, 2011
Online since
December 2010
Export
Price
$32.00
Share

In order to see related information, you need to Login.

In order to see related information, you need to Login.

Authors: Detlef W. Bahnemann, M. Muneer, M. Qamar, M.A. Rahman, H.K. Singh
Abstract:The photocatalysed degradation of various selected pesticide derivatives, namely N,NDimethyl- a-phenyl benzene acetamide (Diphenamid) [1],...
61
Authors: You Rong Duan, W.S. Liu, J. Liu, Z.R. Zhang
Abstract:The objective of this study was to evaluate the in vivo characteristics of poly (ethylene glycol)-poly (lacticacid-co-glycolicacid)-poly...
163
Authors: Hu Yang, Lin Hua Jiang, Yan Zhang
Abstract:The damage process of pure cement pastes with water to cementing material ratios of 0.30, 0.40, 0.50 and 0.60 and fly ash modified pastes...
1162
Authors: Jian Hua Xiang, Chun Liang Zhang, Fan Jiang
Chapter 1: Manufacturing Technology and Processing
Abstract:A spiral micro-groove structure in plane was processed by P-E method in this paper. The shapes of groove structure was restricted by P-E...
346
Authors: Qing Ping Du, Bao Hong Yuan, Ning Zhang, Zhuang Li Lin
Chapter 7: Food Chemistry
Abstract:To study the activating blood and hypolipidemic effects of black mulberry on aged rats, the aged rats were divided into 3 groups and fed with...
1004