Paper Title:
Synthesis and Properties of Fluorescent Biological Molecules Labeled with Novel Silylated Perylene Derivative
  Abstract

We have synthesized novel perylene derivatives bearing a silyl function with modifiable functional groups as a feasible material for visualizing biological substances. The fluorescent perylene derivative was coupled to C-5 position of pyrimidine nucleotide and the nucleotide was subsequently incorporated to the middle position of oligoDNA using an automated DNA synthesizer. Also, the derivative was coupled with a cholesterol analog with different length of linking alkyl function. The resulting modified fluorescent oligoDNA exhibited a small change in its fluorescent signal upon hybridization with the complementary oligoDNA. While the cholesterol analogs bearing the silylated perylene exhibited marked fluorescent signal in living cells. The signal is stronger in the analog having longer linker function compared to the analog having shorter linker function. In each case, however, the signal was much prominent compared to that of dehydroergosterol (DHE), a fluorescent cholesterol analog widely used in biological study.

  Info
Periodical
Edited by
Osamu Hanaizumi and Masafumi Unno
Pages
63-66
DOI
10.4028/www.scientific.net/KEM.459.63
Citation
Y. Sato, A. Ozaki, M. Hosaka, T. Moriguchi, K. Shinozuka, "Synthesis and Properties of Fluorescent Biological Molecules Labeled with Novel Silylated Perylene Derivative", Key Engineering Materials, Vol. 459, pp. 63-66, 2011
Online since
December 2010
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