Natural bone is a composite mainly made from nano/micro-structure of hydroxyapatite and collagen fibers. For bone regeneration by tissue engineering, it is important to synthesize three dimension nano-composites with good biocompatibility, high bioactivity and great bonding property as potentially useful scaffold. In this study, we fabricated chitosan nano-nonwoven scaffold via electrospinning and modified chitosan scaffolds by carboxymethylation (CM) to improve the osteoconductive characteristics. Results of FTIR confirmed the existence of carboxymethyl groups of CMChitosan. Moreover by employed mice osteoblast (MC3T3-E1) cell for adhesion, proliferation and differentiation assays, we found that CMChitosan appeared to have effect on the late stages of osteoblast behavior (calcium deposit).