In this study, a total of 5 CFRP-strengthened reinforced concrete beams were tested in flexure based on the independent development prestressing anchorage system. The various variables included bonding or no bonding of the CFRP and the amount of prestressing. The experiments consisted of one control beam, two prestressed CFRP-unbonded beams, and two prestressed CFRP-bonded beams, all the beams were subjected to four-point bending tests. The ultimate load, deformation, and CFRP strain were examined. The aim of this investigation was to study the flexural performance of reinforced concrete members strengthened using CFRP plates, employing different CFRP bonding and prestressing methods. The failure mode of the prestressed CFRP-plated beams was not debonding, but concrete crushing or FRP rupture. For the reinforced concrete members strengthened with externally bonded prestressed CFRP plates, after the debonding of the CFRP plates that occurred in the bonded cases, the behaviour of the bonded CFRP-plated beams changed to that of the unbonded CFRP-plated beams due to the effect of the anchorage system. The ductility indices of the beams in an unbonded system were less than that of those in a bonded system.