To explore the preparation method of self-prepared Mn0.5Zn0.5Fe2O4 magnetic nanoparticles and were prepared employing a reduction co-precipitation method, transmission electron microscope (TEM), energy dispersive X-ray spectroscopy (SEM-EDS)were used to characterize the product; The results indicated that the Mn0.5Zn0.5Fe2O4 magnetic nanoparticles were prepared successfully. EDS showed each Mn0.5Zn0.5Fe2O4 magnetic nanoparticles contained O, Mn, Zn and Fe elements. The result of heating test showed that Mn0.5Zn0.5Fe2O4 can serve as a heating source upon AMF exposure leading the magnetic nanoparticles to reach its phase transition temperature (41-47.5°C).To evaluate the biocompatibility of Mn0.5Zn0.5Fe2O4 magnetic nanoparticles, which has the potential application in tumor hyperthermia.In the following study it will be used as drug and gene vector, so the compatibility of it should be examined. Then the use acute toxicity (by the mouse of LD50 test), micronucleus (MN) test and hemolysis test were presented to evaluate the toxicity of it in vivo . Its LD50 arrived at 7.1862 g/kg;Micronucleus test showed micronucleus rate of experiment groups had no significant difference compared with negative group. It has no hemolytic .