Hip joint simulator is a device, which is used to investigate the mechanical and frictional wear properties of the biological prosthesis under simulating physiological environment of human hip joint. The simulator is very useful for the final clinical application of the prosthesis. In this research, a data acquisition and control system, which was based on the Digital Signal Processor (DSP) and LabVIEW serial communication technology, was designed for the hip joint simulator. In order to monitoring the simulating mode of motion and stress state of prosthesis, the pressure stepping motor and the angle stepping motor were controlled by the micro-stepping step motor controllers. The angular displacement and pressure signals were collected by the sensors, respectively. Thus, the motion and stress state curves were abtained. This system provided a more accurate platform for in vitro study of the tribological properties of hip joint prosthesis.