Laminated glass and photovoltaic laminated glass are widely used in architecture. The interfacial bonding strengths between poly(vinyl butyral) (PVB) and glass were investigated by the cross-bonding method from room temperature to -50 °C. The loading speed was 5 mm/min, and the cooling speed was about 0.5 °C/min. The testing sample was hold at each temperature for half an hour. It was revealed that the testing temperature had great effect on the bonding strength. At room temperature, the tensile bonding strength was 11.49 MPa and the shear bonding strength was 6.61 MPa. With the temperature decreased from RT to -50 °C, the tensile bonding strength was decreased by 66.81%, but the shear bonding strength was increased by 212.16%. From RT to -30 °C, the change rates of the tensile and shear bonding strength bonding strength were 65.57% and 172.68% respectively, only 3.61% and 14.48% from -30 °C to -50 °C. The mechanism for the bonding strength depended on testing temperatures from RT to -50 °C was also discussed.