In Vivo and In Vitro Biocompatibility Study of Nanostructured Carbonate-Apatite

Rossi, Antonella M.; Fernandes, G.V.O.; Alves, Gutemberg Gomes; Granjeiro, J.M.; Calasans-Maia, M.; Barros, E.; Alvarenga, J.; Canabarro, B.

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Paper Titles

Raman and Infrared Studies of Substituted β-TCP, p.225
The Effect of Microwave Irradiation on Structural and Mechanical Properties of Nano-Structured Bone-Like Carbonated Hydroxyapatite, p.231
In Vivo Behaviour of the Synthetic Porous Hydroxyapatite Prepared by Low Temperature Microwave Processing and Comparison with Deproteinized Bovine Bone, p.236
Standardized Study of Carbonate Apatite as Bone Substitute in Rabbit’s Tibia, p.242
In Vivo and In Vitro Biocompatibility Study of Nanostructured Carbonate-Apatite, p.247
Osseoinduction Evaluation of Hydroxyapatite and Zinc Containing Hydroxyapatite Granules in Rabbits, p.252
Effect of Carbonate-Apatite on Bone Repair in Non-Critical Size Defect of Rat Calvaria, p.258
Study of Bone Repair in Rat Dental Socket after Implantation of Porous Granules of Beta-Tricalcium Phosphate (β-TCP) and Magnesium-Substituted Beta-Tricalcium Phosphate (β-TCMP), p.263
Study of the Release Potential of the Antibiotic Gentamicin from Microspheres of BCP, p.269

Home > Key Engineering Materials > Bioceramics 23 > In Vivo and In Vitro...

Paper Title:

In Vivo and In Vitro Biocompatibility Study of Nanostructured Carbonate-Apatite

Periodical	Key Engineering Materials (Volumes 493 - 494)
Main Theme	Bioceramics 23
Edited by	Eyup Sabri Kayali, Gultekin Goller and Ipek Akin
Pages	247-251
DOI	10.4028/www.scientific.net/KEM.493-494.247
Citation	E. Barros et al., 2011, Key Engineering Materials, 493-494, 247
Online since	October, 2011
Authors	E. Barros, J. Alvarenga, Gutemberg Gomes Alves, B. Canabarro, G.V.O. Fernandes, Antonella M. Rossi, J.M. Granjeiro, M. Calasans-Maia
Keywords	Carbonate Apatite, Hydroxyapatite (HAP), In Vitro, In Vivo
Price	US$ 28,-

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Abstract

The objective of this study was to investigate the in vitro and in vivo biological responses to carbonate apatite (cHA) in comparison to hydroxyapatite (HA). Spheres (400<ø>500 μm) of both materials were synthesized under 5ºC (cHA) and 90ºC (HA) and not sintered. The in vitro cytocompatibility was determined by the XTT assay, according to ISO 10993-5:2009, after exposure of MC3T3-E1 cells to the materials extracts. Ethics Commission on Teaching and Research in Animals approved this project (CEPA/NAL 193/10) and, subsequently, the biomaterials were grafted in the subcutaneous tissues of mice (n=15). After 1 and 3 weeks, five animals of each group were killed for samples removal containing biomaterials and surrounding tissues for histological examination. Semi-serial (5-μm thick) sections were cut and stained with Hematoxylin and Eosin (HE) and the presence of inflammatory infiltrates and biomaterials resorption were evaluated. The experimental group of 3 weeks didn’t show the presence of spheres of both biomaterials and few spheres were observed after 1 week. Histological analysis showed the granulation tissue around the biomaterials with the presence of multinucleated giant cells. After 3 weeks it was observed the presence of fibrous tissue around biomaterials and few inflammatory cells. No signals of tissue necrosis were observed in both groups in all experimental studied periods. Nanostructured carbonate apatite spheres are cytocompatible, biocompatible and present initial biosorption on the subcutaneous comparable to stoichiometric HA, indicating its suitability for further studies on regenerative medicine.