Paper Title:
Biochemical Studies of the Potential Anti-Tumor Activity of Novel Chelate-Setting Apatite Cements
  Abstract

The anti-tumor activity of hydroxyapatite (HAp) cements, which had been developed using a novel setting mechanism termed chelate bonding, against the human osteosarcoma cell line (HOS) and rat bone marrow stromal cells (BMSC) was examined. We aimed to understand the mechanism of the anti-tumor activity of the cement, thereby facilitating improvement of its biological activity. HAp powders were surface-modified with three different concentrations of inositol hexaphosphate (IP6), which were then used to fabricate three different IP6-HAp cements. The amount of IP6 that was bound to the HAp powder, and the amount that was released from the HAp cement, was measured for each sample. Approximately 1/200 to 1/1600 of the bound IP6 was released into the culture medium by day 4. Surface-modification of HAp with high concentration of IP6 inhibits the proliferation of both HOS cells and BMSCs, and appears to induce their apoptotic cell death. HOS cells were slightly more sensitive to IP6 than BMSCs. Thus, novel, chelate-bonded HAp cements are a candidate bone substitution material that exhibit anti-tumor activity.

  Info
Periodical
Key Engineering Materials (Volumes 493-494)
Main Theme
Edited by
Eyup Sabri Kayali, Gultekin Goller and Ipek Akin
Pages
315-319
DOI
10.4028/www.scientific.net/KEM.493-494.315
Citation
H. Konishi, M. Honda, M. Aizawa, N. Kanzawa, "Biochemical Studies of the Potential Anti-Tumor Activity of Novel Chelate-Setting Apatite Cements", Key Engineering Materials, Vols. 493-494, pp. 315-319, 2012
Online since
October 2011
Export
Price
$32.00
Share

In order to see related information, you need to Login.

In order to see related information, you need to Login.

Authors: K. Kida, Y. Horiguchi, Kazuya Oribe, H. Morisue, Morio Matsumoto, Y. Toyama, Mamoru Aizawa
Abstract:We have successfully developed novel “chelate-setting apatite cement” using hydroxyapatite (HAp) particles surface-modified with inositol...
335
Authors: Zilgma Irbe, Linda Vecbiskena, Liga Berzina-Cimdina
Abstract:In this work properties of potential brushite (CaHPO4•2H2O) and hydroxyapatite (Ca10(PO4)6(OH)2) compound cements are investigated. Calcium...
239
Authors: Toshiisa Konishi, Shuhei Takahashi, Minori Mizumoto, Michiyo Honda, Koki Kida, Yukiko Horiguchi, Kazuya Oribe, Ken Ishii, Hikaru Morisue, Yoshiaki Toyama, Morio Matsumoto, Mamoru Aizawa
III. Cements
Abstract:We have developed novel calcium-phosphate cements (CPCs) based on the chelate-setting mechanism of inositol phosphate (IP6) using...
167
Authors: Michiyo Honda, Toshiisa Konishi, Minori Mizumoto, Mamoru Aizawa
III. Cements
Abstract:Cancers frequently metastasize to bone, where it leads to secondary tumor growth, and osteolytic bone degradation. Bone metastases are often...
173
Authors: Yusuke Nakashima, Michiyo Honda, Toshiisa Konishi, Minori Mizumoto, Mamoru Aizawa
III. Cements
Abstract:In our previous study, silicon-containing hydroxyapatite (Si-HAp) powder was prepared via an aqueous precipitation reaction. The Si-HAp...
183