In most biphasic composite systems consisting of sol-gel derived bioactive glass and a second system that is usually used as a reinforcing agent, thorough stirring is necessary to prevent the precipitation of the grains of the second system. Consequently, the aim of this work is to investigate the impact of various stirring rates on the crystallinity and bioactivity of a bioactive glass in the system 58S. Sol-gel-derived bioactive glass (58S) was produced as described in literature. During the gelation, stirring rates of 0, 200, 400, 600 and 800 rpms were applied producing, respectively, the corresponding glass powders. The in vitro bioactivity of the powders was tested in Simulated Body Fluid (SBF) for various immersion times, while the solution was renewed after 6h, 24h and then every 2 days. Fourier Transform Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM) and X-ray Diffractometry (XRD) were used to characterize all materials before and after immersion in SBF solution. FTIR and XRD measurements of all powders revealed mainly the formation of an amorphous glass, while the main crystalline phase was identified to be Ca2SiO4. After immersion in SBF solution for 12h, SEM microphotographs revealed apatite formation on the surface of all samples, while FTIR and XRD confirmed the aforementioned findings. Furthermore, since EDS analysis proved a mean molar Ca/P ratio of about1.7 after 6 days of immersion of all samples- besides those stirred at 400 and 600rpm- it can be assumed that a thick apatite layer was formed covering the whole surface.