Paper Title:
TEM Observation of Biomolecules on/in Mesoporous SBA-15
  Abstract

The encapsulation and immobilization of biomolecules on/in mesoporous silica materials (MPS) have been studied by using transmission electron microscopy (TEM). The mixture of tetraethyl orthosilicate (TEOS) and the triblock copolymer as a template was stirred, hydrothermally treated to form the mesoporous SBA-15 structure, and heat-treated at 550 °C. SEM observation indicated that long and narrow particles were linked together in the long axis direction to form secondary particles of SBA-15. UV-spectrometry was performed to determine the amount of bovine serum albumin (BSA), cytochrome C, myoglobin or -lactoglobulin encapsulated on/in SBA-15. The time profiles for adsorption of proteins can be well described by intraparticle diffusion model. The TEM observations of proteins on/in mesoporous SBA-15 revealed that proteins were embedded on/in mesoporous SBA-15 and the protein behaviors given by TEM observations may correspond to the intraparticle diffusion model.

  Info
Periodical
Key Engineering Materials (Volumes 493-494)
Main Theme
Edited by
Eyup Sabri Kayali, Gultekin Goller and Ipek Akin
Pages
728-731
DOI
10.4028/www.scientific.net/KEM.493-494.728
Citation
Y. Yokogawa, A. Saito, N. Ogawa, A. Nakamura, I. Kishida, "TEM Observation of Biomolecules on/in Mesoporous SBA-15", Key Engineering Materials, Vols. 493-494, pp. 728-731, 2012
Online since
October 2011
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