Paper Title:
Stability of the Magnesium Carbonate Apatite/Anionic Collagen Scaffolds: Effect of the Cross-Link Concentration
  Abstract

Natural bone constitutes of an inorganic phase (a biological nanoapatite) and an organic phase (mostly type I collagen). The challenge is to develop a material that can regenerate lost bone tissue with degradation and resorption kinetics compatible with the new bone formation. The aim of this study was to prepare self-organized magnesium and carbonate substituted apatite/collagen scaffolds, cross-linked with glutaraldehyde (GA). Bovine tendon was submitted to alkaline treatment resulting in a negatively charged collagen surface. The scaffolds were prepared by precipitation: simultaneous dropwise addition of solution containing calcium (Ca) and magnesium (Mg) ions and collagen into a buffered solution containing carbonate and phosphate ions in reaction vessel maintained at 37 °C, pH=8. The reaction products were cross-linked with 0.125 and 0.25% (v/v) glutaraldehyde (GA) solution and freeze-dried. The samples were characterized by Fourier-transformed infrared spectroscopy (FTIR). In vitro cytotoxicity (based on three parameters assays) and scaffolds degradation in culture medium and osteoblastic cells culture were performed in the cross-linked materials. No cytotoxic effects were observed. The cross-linked samples with the lower GA concentration showed a lower stability when placed in contact with culture medium. Human osteoblasts attached on the scaffolds surface cross-linked with 0.25% GA, forming a continuous layer after 14 days of incubation. These results showed potential application of the designed scaffolds for bone tissue engineering.

  Info
Periodical
Key Engineering Materials (Volumes 493-494)
Main Theme
Edited by
Eyup Sabri Kayali, Gultekin Goller and Ipek Akin
Pages
844-848
DOI
10.4028/www.scientific.net/KEM.493-494.844
Citation
M. S. Sader, G. Alves, R. Z. LeGeros, G. D. de Almeida Soares, "Stability of the Magnesium Carbonate Apatite/Anionic Collagen Scaffolds: Effect of the Cross-Link Concentration", Key Engineering Materials, Vols. 493-494, pp. 844-848, 2012
Online since
October 2011
Export
Price
$32.00
Share

In order to see related information, you need to Login.

In order to see related information, you need to Login.

Authors: A.L. Oliveira, Manuela E. Gomes, P.B. Malafaya, Rui L. Reis
101
Authors: A.L. Oliveira, M.I. Santos, A.A. Pedro, Rui L. Reis
Abstract:In the present study sodium clodronate, a well known therapeutic agent from the family of bisphosphonates (BP) was incorporated in an apatite...
615
Authors: Atsushi Sugino, Toshiki Miyazaki, Chikara Ohtsuki, Masao Tanihara, Koichi Kuramoto
Abstract:Natural bone has excellent mechanical properties such as high fracture toughness and high flexibility. These properties are achieved by...
675
Authors: Si Yu Ni, Jiang Chang, Kai Li Lin, Wan Yin Zhai
Abstract:In this study, CaSiO3 (CS)/Ca3(PO4)2 (TCP) composites with 50% CS and 50% TCP sintered at different temperatures (1100oC, 1200oC and 1300oC)...
451
Authors: Yoshitomo Honda, Shinji Kamakura, Kazuo Sasaki, Takahisa Anada, Taisuke Masuda, Osamu Suzuki
479