Paper Title:
Development of an Electrochemical Maltose Biosensor
  Abstract

In this work, electrochemical maltose biosensors based on mutants of the maltose binding protein (MBP) are developed. A rutheniumII complex (RuII), which is covalently attached to MBP, serves as an electrochemical reporter of MBP conformational changes. Biosensors were made through direct attachment of RuII complex modified MBP to gold electrode surfaces. The responses of some individual mutants were evaluated using square wave voltammetry. A maltose-dependent change in Faradic current and capacitance was observed. It is therefore demonstrated that biosensors using generically this family of bacterial periplasmic binding proteins (bPBP) can be made lending themselves to facile biorecognition element preparation and low cost electrochemical transduction.

  Info
Periodical
Edited by
E. Hristoforou and D.S. Vlachos
Pages
116-119
DOI
10.4028/www.scientific.net/KEM.495.116
Citation
C. Morón, E. Tremps, A. Garcia, J. A. Somolinos, "Development of an Electrochemical Maltose Biosensor", Key Engineering Materials, Vol. 495, pp. 116-119, 2012
Online since
November 2011
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$32.00
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