Paper Title:
Synthesis and Properties of Molecular Beacon DNA Probe Bearing Novel Silylated Pyrene Derivative
| Periodical | Key Engineering Materials (Volume 497) |
|---|---|
| Main Theme | Silicon Science and Advanced Micro-Device Engineering II |
| Edited by | Osamu Hanaizumi, Masafumi Unno and Kenta Miura |
| Pages | 47-50 |
| DOI | 10.4028/www.scientific.net/KEM.497.47 |
| Citation | M. Gias Uddin et al., 2011, Key Engineering Materials, 497, 47 |
| Online since | December, 2011 |
| Authors | M. Gias Uddin, Tomohisa Moriguchi, Mayumi Ichimura, Kazuo Shinozuka |
| Keywords | Excimer, Molecular Beacon, Pyrene, Silylation |
| Price | US$ 28,- |
View full size
Abstract
A novel stem-loop structured fluorescent oligoDNA probe (molecular beacon probe) bearing a silylated pyrene derivative at C-5 position of deoxyuridine has been synthesized. The fluorescently labeled modified nucleoside has been incorporated into two consecutive positions in the stem segment of the DNA using an automated DNA synthesizer. The resulting modified DNA exhibited an excimer fluorescent signal upon binding to the fully matched complementary DNA strand. The excimer emission was, however, effectively quenched while it stays alone or it hybridizes to a single base mismatched complementary target.
