Paper Title:
Synthesis and Properties of Molecular Beacon DNA Probe Bearing Novel Silylated Pyrene Derivative
  Abstract

A novel stem-loop structured fluorescent oligoDNA probe (molecular beacon probe) bearing a silylated pyrene derivative at C-5 position of deoxyuridine has been synthesized. The fluorescently labeled modified nucleoside has been incorporated into two consecutive positions in the stem segment of the DNA using an automated DNA synthesizer. The resulting modified DNA exhibited an excimer fluorescent signal upon binding to the fully matched complementary DNA strand. The excimer emission was, however, effectively quenched while it stays alone or it hybridizes to a single base mismatched complementary target.

  Info
Periodical
Chapter
Chapter 2: Chemical Science and Technology
Edited by
Osamu Hanaizumi, Masafumi Unno and Kenta Miura
Pages
47-50
DOI
10.4028/www.scientific.net/KEM.497.47
Citation
M. G. Uddin, T. Moriguchi, M. Ichimura, K. Shinozuka, "Synthesis and Properties of Molecular Beacon DNA Probe Bearing Novel Silylated Pyrene Derivative", Key Engineering Materials, Vol. 497, pp. 47-50, 2012
Online since
December 2011
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Price
$32.00
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