Taxus chinensis var. maire is an important medicinal, ecological and environmental friendly plant. To propose calculation methods of three-dimensional ecological benefit assessment index of Taxus chinensis var. maire, 10 main indicators of 90 Taxus chinensis var. maire at the age of 10 to 15 were measured and calculated, including heights of trees and crowns, diameter of crowns and roots, largest vertical section area of crowns, numbers of branches and leaves, length, width and area of leaves, based on consideration of situations such as altitude, coordinates, solar radiation, mean annual sunshine hours, rainfall and temperature, days with 4 or more than 4 power wind, frost-free period, etc. Ecological benefits of Taxus chinensis var. maire were analyzed using two-and three-dimensional models based on the research of three-dimensional vegetation volume, and the main results were analyzed and summarized. This will provide a scientific assessment standard for exploitation and territory plantation of Taxus chinensis var. maire.