The detection of milk proteins can be achieved by light scattering. But in the process of detection, there are many influences that directly affected the accuracy of detection. On the basis of researching the light scattering characteristic of milk proteins, we have studied the effect of physical-chemical conditions such as scattering angle and temperature on scattering light intensity. Through simulation experiments, we can find that parameters such as scattering angle and temperature also have directly impact to the casein micelle detection. According to the simulation result, the detection parameter has been optimized, the theoretical support and guidance for increase the detection accuracy also has been provided.