To design the scaffold with suitable properties for the development of tissue engineered livers, materials design, selection and scaffold construct are three dispensable steps to be followed consequently. Firstly, some natural materials such as collagen, chitosan and alignate as well as some prevailing aliphatic polyester such as poly(lactic acid) (PLA), polyglycolide (PGA) and their copolymers poly(lactide-co-glycolide) (PLGA) are selected and characterized by hepatocyte culture. The experimental results reveal that the natural materials with excellent biocompatibility are not suitable as the scaffold alone because of the poor mechanical properties. At same time, aliphatic polyesters with good mechanical properties and biodegrade abilities are also proved inapplicable for hepatocytes for the lack of right cell recognition sites. Among our study scope, the hybrid materials such as collagen/chitosan or collagen/chitosan/heparin are the very promising candidates for hepatic tissue engineering scaffold. Secondly, two novel designs composed of collagen and PLGA, namely, the spindle porous scaffold with separated channels for transportation of nutrient, plasma and the one made by dewaxen-casting method are initiated respectively.