Paper Title:
Head-On Immobilization of DNA Fragments on CVD-Diamond Layers
  Abstract

Synthetic diamond is regarded as a promising material for biosensors: it forms a stable platform for genetic assays and its biocompatibility opens the possibility for in vivo sensing. In this study the use of a thymidine linker for covalent DNA attachment was evaluated. Contact angle measurements provided a qualitative test of the initially oxidized surface. X-ray photoemission spectroscopy was used for further analysis of the oxides and for monitoring the effect of subsequent chemical treatments. The presence of FITC-labelled DNA was confirmed by confocal fluorescence microscopy. Enzyme linked immunosorbent assays indicated that this DNA was merely adsorbed on the diamond surface instead of covalently bound.

  Info
Periodical
Materials Science Forum (Volumes 492-493)
Edited by
Omer Van der Biest, Michael Gasik, Jozef Vleugels
Pages
267-272
DOI
10.4028/www.scientific.net/MSF.492-493.267
Citation
S. Wenmackers, P. Christiaens, W. Deferme, M. Daenen, K. Haenen, M. Nesládek, P. Wagner, V. Vermeeren, L. Michiels, M. Van de Ven, M. Ameloot, J. Wouters, L. Naelaerts, Z. Mekhalif, "Head-On Immobilization of DNA Fragments on CVD-Diamond Layers", Materials Science Forum, Vols. 492-493, pp. 267-272, 2005
Online since
August 2005
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Price
$32.00
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