The mechanical processing of metals results often in development of preferred orientation of grains or texture. On the other hand, the orientations of grains have a significant effect on the friction and wear behaviour of materials in contact. The objective of this investigation was to determine the crystallographic anisotropy of the coefficient of friction. Medium carbon steel AISI 1045 were selected as the substrate material. Automated orientation imaging analysis system (OIM) was used to identify orientations of grains in polycrystalline specimens. Micro-friction tests were carried out using a Tribocope, a combination of nano-mechanical probe and atomic force microscope with an electrochemical cell. The micro friction tests were performed along different crystallographic directions inside different grains.The obtained results have shown that the coefficient of friction for individual grains depends on crystallographic orientation, and that the coefficient of friction for higher atomic density planes is lower than that for lower atomic density planes.