Paper Title:
A Micromachine-Based Flow Cytometer Chip Integrated with Micro-Pumps/Valves for Multi-Wavelength Detection Applications
  Abstract

This study presents a new micromachine-based cell counting and sorting system capable of multi-wavelength detection for biological applications. Chip-based flow cytometers have been extensively investigated recently. Several unique features have been integrated on this new cell-analysis system. First, a series of new serpentine-shape pneumatic micro-pumps were used to drive sample and sheath flows and generate a hydrodynamic focusing effect. The control of fluids could be achieved with ease by using this approach. Multiple embedded optical fibers were then used to transmit the light source and collect induced fluorescence signals in and out of the chip device. Cells labeled with different fluorescent dyes could be then individually detected by corresponding fibers using lasers with different wavelengths. Finally, three pneumatic micro-valves downstream were used for cell sorting. The developed micro flow cytometer could be used for fast analysis of cell-related bio-medical applications.

  Info
Periodical
Materials Science Forum (Volumes 505-507)
Edited by
Wunyuh Jywe, Chieh-Li Chen, Kuang-Chao Fan, R.F. Fung, S.G. Hanson,Wen-Hsiang Hsieh, Chaug-Liang Hsu, You-Min Huang, Yunn-Lin Hwang, Gerd Jäger, Y.R. Jeng, Wenlung Li, Yunn-Shiuan Liao, Chien-Chang Lin, Zong-Ching Lin, Cheng-Kuo Sung and Ching-Huan Tzeng
Pages
637-642
DOI
10.4028/www.scientific.net/MSF.505-507.637
Citation
C. M. Chang, S.K. Hsiung, G. B. Lee, "A Micromachine-Based Flow Cytometer Chip Integrated with Micro-Pumps/Valves for Multi-Wavelength Detection Applications", Materials Science Forum, Vols. 505-507, pp. 637-642, 2006
Online since
January 2006
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Price
$32.00
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