Paper Title:
The Transfection Efficacy of Chitosan-DNA Nanoparticles on MGC803, SMMC-7721, MG63 Cells
  Abstract

Chitosan-DNA nanoparticles were synthesized from the complexation of chitosan solution and the plasmid pCD-GFP encoding green fluorescent protein through a coacervation process. The nanoparticles formed were of diameter inferior to 100 nm. The transfection efficacy of chitosan-DNA nanoparticles in MGC803 cells was higher than in SMMC-7721 cells, whereas the plasmid GFP was not expressed in MG63 cells.

  Info
Periodical
Materials Science Forum (Volumes 510-511)
Edited by
Hyung Sun Kim, Yu Bao Li and Soo Wohn Lee
Pages
382-385
DOI
10.4028/www.scientific.net/MSF.510-511.382
Citation
S. Chen, B. Zhang, L. Ren, L.-P. Sun, Q. Q. Zhang, "The Transfection Efficacy of Chitosan-DNA Nanoparticles on MGC803, SMMC-7721, MG63 Cells", Materials Science Forum, Vols. 510-511, pp. 382-385, 2006
Online since
March 2006
Export
Price
$32.00
Share

In order to see related information, you need to Login.

In order to see related information, you need to Login.

Authors: Hyun Jung Lee, Keun Hong Park, So Ra Park, Byoung Hyun Min
Abstract:Acknowledging the supportive influence of matrix molecules on the chondrocytic phenotype, we combined heparin and chitosan to develop a...
329
Authors: Anna Theocharidou, K. Tsoptsias, Eleana Kontonasaki, Lambrini Papadopoulou, C. Panayiotou, Konstantinos M. Paraskevopoulos, Petros Koidis
Abstract: Chitin is a polysaccharide abundant in nature. Its’ deacetylation product-chitosan- in combination with gelatin (collagen product) is...
866
Authors: Hong Lian Guo, Hai Yang Zhao, Yuan Yuan Sun
Chapter 2: Biology Technology and Materials
Abstract:Oligosaccharide as elicitor signal that induced the plant epidermal cells early apoptosis and death events were investigated In this...
805
Authors: A. Apirakaramwong, Perayot Pamonsinlapatham, S. Techaarpornkul, Praneet Opanasopit, Suwannee Panomsuk, S. Soksawatmaekhin
III. Applications of Biomaterial
Abstract:Chitosan (CS) has a high potential for gene delivery into mammalian cells. However, its uptake mechanism is not well clarified. We...
485
Authors: Song Chen, Nobutaka Hanagata
Chapter 1: Advanced Bio-, Medical-Material Technology and Application
Abstract:Silica-chitosan hybrid was synthesized from a mixture of chitosan and glycidyloxypropyltrimethoxysilane and then coated on a microgrooved...
165