Ti-6.5Al-2Zr-1Mo-1V is a near alpha titanium alloy strengthened by solid solution with Al and other components. In this study, a series of experiments on tool wear and surface integrity in high speed milling (HSM) of this alloy were carried out. The tool lives under different cutting speeds were studied and the corresponding empirical equation of tool life was derived. Additionally, the wear mechanism of cutting tools was also discussed. Finally, surface integrity, including surface roughness, metallograph, work hardening and residual stresses, were examined and analysed. The result shows that good surface quality of workpiece could be obtained in HSM of the alloy.