Charpy transition temperature TCVN and reference temperature To for 10Ni3CrMoV steel were determined using two different experimental techniques such as Charpy V-notch impact test technique and reference temperature To test technique. It was found that two methods provided different test results. The median master curve with upper and lower tolerance bounds was got from the test. The upper tolerance bound is often used for material design and application. At the same time the TCVN and To results were discussed for two kinds of heat treatment which are QT(Quenching and Temper) and QLT(Quenching, anneal and temper). JC (med ) K values calculated were 101 and 105MPam1/2 for the QT and QLT steels, respectively. These indicated that the QLT steels have the higher JC (med ) K , the lower reference temperature and lower energy (or fracture appearance) transition temperature(ETT50 or FATT50) than the QT steels. This was mainly related with the different microstructures of two kinds of heat treatment.