Nano-hydroxyapatite/polyamide66/chitosan composite (n-HA/PA66/CS) was prepared by a co-precipitation method and porous scaffolds from n-HA/PA66/CS composite were prepared by means of porogen–leaching method and were characterized by SEM, IR, XRD and universal mechanical testing machine. IR and XRD analyses showed that some chemical bonds existed between n-HA and polymers. Furthermore, macroporous structure of the scaffolds and mechanical strength were tested with a changed ratio of porogens (PVP/NaCl). When the ratio of PVP and NaCl is1: 6, the scaffold processed highly porosity and the pores were interconnected. The compressive strength of the scaffold, can meet the requirement of tissue regeneration.