The effect of heat treatment on microstructures and stress rupture property of a Ni3Al base single crystal alloy DDIC6 was studied in the present investigate. The single crystal specimens were produced by screw selection crystal method. The heat treatment for the alloy was 1300°C/10h+1120°C/4h+870°C/32h and 1300°C/10h+870°C/32h.The microstructures were examined by SEM, TEM and X-ray EDS techniques. The stress rupture tests were carried out in air by constant load creep machines under 1100°C/130MPa with the specimens size of φ5×25 mm. The experimental results showed that the as-cast large size γ′ phases entirely dissolved after 1300°C/10h, and secondary fine γ′ phases precipitated by following aging at 1120°C and 870°C for certain periods of time. The stress rupture life under 1100°C/130MPa increased from 20~30hrs for as-cast condition to 60~100hrs for heat treatment condition. The improvement of the creep resistance of the alloy may attribute to the decrement of the elements segregation at dendrite and interdendritic areas, and the proper size and distribution of γ′ phases.