Paper Title:
Silk as Substratum for Cell Attachment and Proliferation
  Abstract

Silk fibroin (SF), isolated from silkworm (Bombyx mori) cocoons, is a natural biodegradable polymer. Over the past decade, there was some interest in using SF as a biomedical material. As part of a project to develop tissue-engineered constructs for the surgical restoration of the ocular surface (cornea, conjunctiva), we have investigated the capacity of SF to function as a substratum for the attachment and growth of corneal stem/progenitor cells harvested from the corneoscleral limbus of donor human corneal tissue. SF membranes were produced from cocoons following a protocol involving successive dissolution steps, filtration, dialysis, evaporation, and methanol treatment. Human limbal epithelial cells were harvested from donor tissue and seeded onto SF membranes. After 5 days, the culture was fixed and stained with specific agents to visualize the cells. The study indicated profuse cellular attachment and growth. SF membranes appear to be suitable as a substratum for the repair of damaged ocular surface.

  Info
Periodical
Materials Science Forum (Volumes 561-565)
Main Theme
Edited by
Young Won Chang, Nack J. Kim and Chong Soo Lee
Pages
1549-1552
DOI
10.4028/www.scientific.net/MSF.561-565.1549
Citation
T. V. Chirila, Z. Barnard, Z. Zainuddin, D. Harkin, "Silk as Substratum for Cell Attachment and Proliferation", Materials Science Forum, Vols. 561-565, pp. 1549-1552, 2007
Online since
October 2007
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$32.00
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