TiO2 / KGM composite membranes were prepared by blend. Effects of the content of TiO2 and glycerol, ratio of PVA to KGM on tensile strength of membranes were investigated. Structure and Morphology of TiO2 / KGM films were characterized by SEM, XRD and IR. Furthermore, anti-bacteria properties of composite films were evaluated. The results showed that there were hydrogen bonding interactions between konjac glucomannan molecule, PVA and titania particle after irradiation. Composite membranes enable the antibacterial properties improved by adding an appropriate amount of TiO2, but mechanical properties decreased. The composite membrane tensile strength decreased with increasing glycerol content but increased with PVA glucomannuronate composite ratio increasing.