Skin wounds, burns and scars represent a major burden upon world healthcare costs. The successful creation in vitro of skin substitutes has been the focus of a concentrated research activity for the last 30 or more years, culminating in some success for its translation into the clinical setting. In this paper, a novel artificial skin of bacterial cellulose has been biosynthesized by Acetobacter xylinum and modified by chitosan. BC is thought as an optimal substrate material of artificial skin. It is thought to be an interesting material for using as a substratum of artificial skin because it could provide moist environment to a wound resulting in a better wound healing. But BC has no antimicrobial activity to reduce the probability of wound infection. To achieve antimicrobial activity of BC, it was modified by chitosan due to its antibiotic activity. The structure and components were characterized by FTIR and ESEM. The results indicated the nano-composites of BC and chitosan are promising and potential for wound dressings.