PPy film with micro-antennal morphology was prepared by potentio-static method without any templates directly onto the surfaces of iron, platinum and gold-plated plastic sheets, respectively. Morphology of PPy film was observed with scanning electron microscopy, and its electrochemical properties were determined by means of cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Attention was paid to the influence of reaction time, potential value, types of substrates, types and conconcentrations of electrolyte on the morphology and electrochemical properties of PPy film. Stable PPy film with thriftily long antennae was obtained on different substrates with good electrochemical properties. The film was applied for a prototype of biosensor, in which bioactive substance of glucose oxidase was immobilized.