A novel two-step foaming process has been developed to prepare the porous titanium implant. By using H2O2 and stearic acid as foaming reagent ordinally, a foamed structure with an open, interconnected pore morphology was obtained. The mechanical property was determined by compressive test. In vitro study was conducted to evaluate the ability of the porous titanium to support the growth and differentiation of Human osteosarcoma cell line MG63. The results show that the porous titanium has better interconnection compared to that obtained by traditional slurry foaming and its compressive strength and Young’s modulus were approximate 23.6 MPa and 2.1 GPa, respectively. Cell culture experiment results indicate that the porous titanium has good biocompatibility and acid-alkali treatment facilitates the adherence and proliferation of cells.