Paper Title:
Stimuli-Responsive Materials Applied as Carriers of Immobilized Enzymes
  Abstract

Enzymes, immobilized on pH/temperature/ion-responsive carriers, relieve poor contact and could be conveniently recycled as well. Enzymes were immobilized on pH-responsive carriers often by covalent method, which was better than non-covalent method (adsorption). The immobilized enzymes showed slight steric hindrance for enzymatic reaction, but always lose much activity during recycle process. The Km values were often increased significantly via immobilization on temperature/ion responsive carriers, while the immobilized enzymes expressed superior reusability. Both covalent and non-covalent methods were fit for enzymes to be immobilized on temperature-responsive carriers. Non-covalent method (entrapment) was preferential choose for enzymes to be immobilized on ion-responsive carriers. Many drawbacks, such as large activity loss and slow respondence during stimulus occurrence, seriously restrained the effect of the immobilized enzymes. It was supposed to open up special stimuli-responsive materials for enzymes immobilization, and choose proper immobilization methods, correspondingly.

  Info
Periodical
Materials Science Forum (Volumes 610-613)
Main Theme
Edited by
Zhongwei Gu, Yafang Han, Fusheng Pan, Xitao Wang, Duan Weng and Shaoxiong Zhou
Pages
1198-1202
DOI
10.4028/www.scientific.net/MSF.610-613.1198
Citation
Y. Zhang, J. L. Xu, Z. H. Yuan, "Stimuli-Responsive Materials Applied as Carriers of Immobilized Enzymes", Materials Science Forum, Vols. 610-613, pp. 1198-1202, 2009
Online since
January 2009
Export
Price
$32.00
Share

In order to see related information, you need to Login.

In order to see related information, you need to Login.

Authors: Shu Juan Xiao, Shou Wu Yu, Shui Jing Gao, Hong Xia Guo
Chapter 10: Biomaterials, Microbiology and Biotechnology
Abstract:Molecular imprinting technology(MIT)is a kind of new-emerging polymerizing technology with specific selectivity and affinity ability, An...
1678