The gradient silk fibroin (SF)/ hydroxyapatite (HA) porous composite used for the scaffold of cartilage-bone tissue regeneration was prepared with SF powder and HA powder by layer-by-layer mould pressing method and granular NaCl as porogent. The characteristics were performed by Electron Microprobe and Energy Dispersive Spectometer (EDS). It indicated that the distribution of SF and HA presented linear gradient along the thickness direction in the materials. The porosity of composites increased with NaCl content increasing, while the density decreased. As the result of mechanical properties measurement, the bending strength and compressive strength decreased with NaCl content increasing. Compared the mechanical properties between gradient material and non-gradient material, it indicated that bending strength of gradient SF/HA porous material was higher than non-gradient SF/HA porous material’s.