Paper Title:
Construction of Two-Gene Modified Artificial Bone by Using Recombinant Adenovirus
  Abstract

Large osseous defects are difficult to treat because of deficient blood supply on the defected area. To get sufficient blood supply, we designed to establish the adenovirus simultaneously encoding both VEGF and Ang-1 (pAd-VIA) to accelerate the formation of new vessels in the process of bone defect repair. The construction of the adenovirus was performed according to the method reported by Tong-Chuan HE with a tiny modification. Three kinds of adenoviruses were acquired. They are adenovirus pAd-VIA simultaneously encoding VEGF and Ang-1, adenovirus pAd-VEGF encoding VEGF, and adenovirus pAd-Ang-1 encoding Ang-1. The adenovirus prepared in this study could successfully transfer VEGF and Ang-1 into mesenchymal stem cells(MSCs) with high efficiency. Two-gene modified artificial bone was established by use of these adenovirus. In the end, the two-gene modified artificial bone was proved to have good biocompatibility and biological function. Study reports presented here will pave the way for further exploration of vascularization in the process of large osseous defects repair.

  Info
Periodical
Materials Science Forum (Volumes 610-613)
Main Theme
Edited by
Zhongwei Gu, Yafang Han, Fusheng Pan, Xitao Wang, Duan Weng and Shaoxiong Zhou
Pages
1343-1355
DOI
10.4028/www.scientific.net/MSF.610-613.1343
Citation
C. G. Duan, G. L. Meng, J. Liu, Y. Y. Hu, B. F. Li, D. Li, J. P. Bai, L. Bi, M. Liu, "Construction of Two-Gene Modified Artificial Bone by Using Recombinant Adenovirus", Materials Science Forum, Vols. 610-613, pp. 1343-1355, 2009
Online since
January 2009
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Price
$32.00
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