Paper Title:
Effect of Hydroxyapatite Nanoparticles of Different Concentrations on Rat Osteoblast
  Abstract

Nano-HAP (10-20nm) were obtained from East China University of Science and Technology. The osteoblasts were primary cultured from rat calvaria and then treated with five different concentrations(20,40,60,80,100µg/ml) of nano-HAP, the osteoblasts without nano-HAP was used as control group. Inhibition ratio, apoptotic rate were evaluated by MTT assay and flow cytometry (FCM), respectively. The specific surface area of nano-HAP was detected by BET. All date were expressed as mean ± standard deviation.Statistical analysis was performed by t test using software SPSS11.0 for Windows. The results indicated that the nano-HAP could inhibit the growth of osteoblasts in a dose-dependent manner. When the concentrations of nano-HAP were 20, 40, 60, 80, 100µg/ml, the inhibition ratio were 2.8%, 22.2%, 26.9%, 38% and 47.7%, and the apoptotic rate were 4.63%, 6.75%, 9.47%, 11.49%, 17.22%, respectively, which were obviously higher than that of control group. The nano-HAP significantly induced apoptosis in osteoblasts. There were the same tendency that the apoptotic and inhibition ratio of osteoblasts were rising with the increasing of the concentration of the nano-HAP. The specific surface area of nano-HAP was 148.140m2/g.

  Info
Periodical
Materials Science Forum (Volumes 610-613)
Main Theme
Edited by
Zhongwei Gu, Yafang Han, Fusheng Pan, Xitao Wang, Duan Weng and Shaoxiong Zhou
Pages
1364-1369
DOI
10.4028/www.scientific.net/MSF.610-613.1364
Citation
Z. L. Xu, J. Sun, C. S. Liu, J. Wei, "Effect of Hydroxyapatite Nanoparticles of Different Concentrations on Rat Osteoblast", Materials Science Forum, Vols. 610-613, pp. 1364-1369, 2009
Online since
January 2009
Export
Price
$32.00
Share

In order to see related information, you need to Login.

In order to see related information, you need to Login.

Authors: Zhi Wang, Wen Hui Fu, Xiang Yang Lu, Guang Xian Cai
Chapter 2: Smart Materials for Biomedical Engineering Application
Abstract:This paper focuses on the effect of the venom of the scorpion Buthus martensii on the proliferation of human cervical carcinoma Hela...
393
Authors: Qing Qing Chen, Jiao Sun
Chapter 1: Advanced Bio-, Medical-Material Technology and Application
Abstract:Despite applications of Silica nanoparticles (SiO2 NPs) have been developed in the biomedicine field, relatively few studies have been...
42
Authors: Ying Xun Liu, Jian Yuan Huang, Dong Liang Wang, Jin Ke Wang
Chapter 2: Biological and Medical Materials, Technologies and Engineering
Abstract:This study investigated the cell apoptosis and gene expression profiles of human THP-1 monocytes in order to identify the molecular mechanism...
377