An experimental and numerical study of polymer electrolyte membrane fuel cell (PEMFC) is presented and compared with the experimental data to investigate the effects of pressure gradient, flow rate, humidification and supplied oxidant type for the practical application. The membrane and electrolyte assembly (MEA) materials are implemented by double-tied catalyst layers. A single-phase two-dimensional steady-state model is is implemented for the numerical analysis. Testing condition is fixed at 60sccm and 70°C in anode and cathode, respectively. It is found that the performance of PEMFC depend highly on the conditions as gas pressure, temperature, thickness, supplied oxidant type (Oxygen/Air) as well as humidification. The results show that the humidification effect enhances the performance more than 20% and the pure oxygen gas as fuel improves current density more than 25% compared to ambient air suppliance as oxidant.