The effects of post-weld heat treatment on 3.05-mm thick Ti-6Al-4V alloy were investigated using a 4 kW Nd:YAG laser. Two main defects, underfill and porosity, were observed. The use of filler wire reduced underfill defects but slightly increased porosity. No cracks were detected. The as-welded and stress-relief annealed welds had very similar microstructures, hardness, and tensile properties. However, the post-weld solution heat treatment and aging transformed the martensite in the fusion zone into a coarse interlamellar α-β structure, causing a decrease in ductility but a more homogeneous distribution in the hardness of the welds.