Paper Title:
Proliferation and Migration of Human Osteoblasts on Porous Three Dimensional Scaffolds
  Abstract

Porous tantalum (Ta) biomaterial is designed to function as a scaffold for osseous ingrowths and has found applications in orthopedics. Integration of this Ta foam into the neighboring bone requires that osteoprogenitor cells attach to the implant, grow into the scaffold, proliferate and differentiate to osteoblasts. The aim of the present study was to create an in vitro 3D model system to investigate the interaction of human osteoblasts with porous Ta in the depth of the corpus. To explore active migration of osteoblasts into the Ta scaffold two porous Ta discs (Zimmer, Poland) were horizontally fixed within a clamping ring. Thereby a 3D Ta module with 4 levels is generated, which is placed into a cell culture well with the appropriate medium. Osteoblast-like cells were seeded apical onto the Ta module and cultured for 7 days in humidified atmosphere. Active migration of cells into the scaffold was monitored by field emission scanning electron microscopy (FESEM) imaging of the apical, medial and basal layers. A problem in 3D cell culture is the nutrition of cells inside of the scaffold. Therefore morphological changes and differentiation of the cells in distinct layers were analyzed.

  Info
Periodical
Materials Science Forum (Volumes 638-642)
Main Theme
Edited by
T. Chandra, N. Wanderka, W. Reimers , M. Ionescu
Pages
506-511
DOI
10.4028/www.scientific.net/MSF.638-642.506
Citation
C. Bergemann, E. D. Klinkenberg, F. Lüthen, A. Weidmann, R. Lange, U. Beck, R. Bader, K. Schröder, B. Nebe, "Proliferation and Migration of Human Osteoblasts on Porous Three Dimensional Scaffolds", Materials Science Forum, Vols. 638-642, pp. 506-511, 2010
Online since
January 2010
Export
Price
$32.00
Share

In order to see related information, you need to Login.

In order to see related information, you need to Login.

Authors: Ho Yun Chung, Eun Jung Oh, Jin Hyun Choi, Byung Chae Cho
Abstract:Mesenchymal stem cells (MSCs) from bone marrow seem to be the one of best candidates to regenerate injured tissue. However, recent advances...
193
Authors: Lia Rimondini, Federica Demarosi, Ismaela Foltran, Nadia Quirici
Chapter 3: Biomaterials
Abstract:Electrospinning technique is an efficient processing method to manufacture micro-and nanosized fibrous structures by electrostatic force for...
584
Authors: Miho Nakamura, Akiko Nagai, Kimihiro Yamashita
VI. Cell Studies and Cell-Material Interactions
Abstract:The osteoblast behaviors on the biomaterial substrates are recognized to play a fundamental role in osteoconduction process. The purpose of...
357
Authors: Wei Chung Liu, Chang Shu Tsai, Ya Yun Chen, Nien Tzu Keng
Chapter 2: Advanced Bio and Medical Engineering, Applications of Bioinformatics and Genetic Engineering
Abstract:Silica containing materials are often applied in bone tissue engineering, which may contact with bone marrow cells. However, the biological...
494
Authors: Masanobu Kamitakahara, Takashi Shirato, Taishi Yokoi, Hideaki Matsubara, Yasuaki Shibata, Tohru Ikeda
Chapter 3: Cell Response to Bioceramic Characteristics In Vitro
Abstract:Silicate-containing alpha-tricalcium phosphate (α-TCP) ceramics are expected to be useful scaffolds for bone regeneration because α-TCP shows...
90