Paper Title:
A Dual Role of Copper on the Surface of Bone Implants
  Abstract

To stimulate bone regeneration, the design of bioactive implants is a great challenge in current orthopedic research. We reasoned that implants should be suitable both to stimulate osteogenic differentiation of mesenchymal stem cells and prevent infections at the site of implantation. Therefore, we focus on copper ions, which are known to exert antimicrobial effects. On the other hand, copper is essential for the cell physiology, including the formation of the extracellular matrix. We studied the influence of copper ions on mesenchymal stem cells at various concentrations and identified the limits of copper concentrations for cell survival. Below the critical concentration for cell survival we analysed proliferation and osteogenic differentiation of the cells in the presence of copper ions. We found that copper stimulated the proliferation of the mesemchymal stem cells at 0.1 mM. Osteogenic differentiation decreased after 14 days at a concentration of 0.05 - 0.1 mM copper ions in osteogenic medium measured by the expression of osteogenic proteins, like alkaline phosphatase (ALP), bone sialoprotein (BSP) and collagen I (COL). We argue that at the implant surface a higher concentration of copper could prevent biofilm formation of bacteria and physiological concentrations in the vicinity of the implant would stimulate stem cell expansion. Together, copper is an interesting agent to control both bacteria and stem cells in the field of implant technology.

  Info
Periodical
Materials Science Forum (Volumes 638-642)
Main Theme
Edited by
T. Chandra, N. Wanderka, W. Reimers , M. Ionescu
Pages
600-605
DOI
10.4028/www.scientific.net/MSF.638-642.600
Citation
F. Lüthen, C. Bergemann, U. Bulnheim, C. Prinz, H. G. Neumann, A. Podbielski, R. Bader, J. Rychly, "A Dual Role of Copper on the Surface of Bone Implants", Materials Science Forum, Vols. 638-642, pp. 600-605, 2010
Online since
January 2010
Export
Price
$32.00
Share

In order to see related information, you need to Login.

In order to see related information, you need to Login.

Authors: Christine Knabe, C. Rolfe Howlett, Boban Markovic, Georg Berger, Renate Gildenhaar, Hala Zreiqat
679
Authors: Hala Zreiqat, Georg Berger, Renate Gildenhaar, Christine Knabe
1059
Authors: Jun Yao, Shula Radin, Gwendolen Reilly, Phoebe S. Leboy, Paul Ducheyne
Abstract:A previous study demonstrated that the incorporation of bioactive glass (BG) into poly (lactic-co-glycolic acid) (PLGA) can promote the...
619
Authors: Xia Lu, Li Ang Xing, Pei Zhi Wang, Jun Fu
Abstract:The aim of this study is to fabricate an implant framework for tissue engineering by sintering titanium beads and coating with...
613
Authors: Christine Knabe, Marco Lopez Heredia, Dirk Barnemitz, Antje Genzel, Fabian Peters, Wolf Dietrich Hübner
I. Bioceramics Synthesis, Characterization and Testing
Abstract:This study evaluates the effect of two novel particulate silicon-doped calcium phosphate graft materials as compared to the currently...
31